Title : Synthesis optimalization and release characterization of the drug-loaded poly(lactide-co-glycolide) colloidal particles
Abstract:
Nowadays, the polymer-based nanostructured drug carriers play a key role in the field of na-nomedicine. By using them, we can prolong and increase the efficacy of the drugs. Due to the excellent biocompatibility and good material properties, the poly(lactide-co-glycolide) (PLGA) copolymers is one of the most popularly used biopolymers in the development of new type of colloidal drug delivery systems. The degradation and hydrophilicity properties of PLGA are excellently controllable with the changing of the monomer ratios, which is advantageous in the application of these carriers. Our aim was to prepare drug-containing nanoparticles (NPs) by using poly(lactide) (PLA) and PLGA and to determine the effect of the synthesis parameters (e.g concentration, solvent and stabilizing agents) on the structure of the NPs. Nanoprecipitation method was used for the preparation of these systems, where the NPs have been characterized by DSC, FT-IR spectroscopy, DLS, TEM, SEM and UV-Vis measurements. During the synthesis of the PLGA NPs, it has been proved that the hydrodinamic diameter and the size distribution of the particles can be controlled with the changing of the initial parameters. The drugs having different hydrophilicity (e.g. ketoprofen (KP), D-α-tocopherol polyethylene glycol 1000 succinate (TPGS) and (±)-α-tocopherol (TP)) have been encapsulated, where the highest entrapment efficiency (%) is obtained for the most lipophilic (±)-α- tocopherol (~90%). For the characterization of the drugs release properties, we investigated the dissolution profiles of the drug-loaded PLGA NPs. Based on the results, we found that the release of the active substance can be changed by the lactide:glycolide ratio. For the determination of the kinetic parameters, the release curves were fitted by different kinetic model using nonlinear regression. We can defined that the release of the drugs meanly have diffusion controlled feature.
Audience Take Away Notes:
- The presentation will be highlighted to the advantages of the polymer-based drug delivery systems and importance of these systems in the field of the nanomedicine
- The audience can get to know the effect of the initial parameters to the preparation of the PLGA-based particles
- Results of the in vitro release measurements are pointed out, how can be changed the dissolution kinetic properties of the drug-loaded PLGA at different conditions, which knowledge is essential for subsequent in vivo studies
